General Information of DFM (ID: F13708)
Name	Metolazone 2.5 mg tablet
Company	Carilion Materials Management
Active Pharmaceutical Ingredient (API)	Metolazone		API Info click to show the detail info of this API
	Congestive heart failure		ICD-11: BD10	Approved
Drug Inactive Ingredient (DIGs)
	DIG ID	DIG Info	DIG Name	DIG Functional Class
	E02QDK	DIG Info	D&C red no. 33	Colorant
	E01UTP	DIG Info	Magnesium stearate	lubricant

Full List of Biological Targets of DIG (DBTs) Regulated by DIG(s) in This DFM
Oxidoreductase (ORase)
DBT Name: Albendazole monooxygenase (CYP3A4)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Magnesium stearate
Biological Activity	Protein expression downregulation (tested by experiment)					[1]
Tested Species	Homo sapiens (Human)
UniProt ID	CP3A4_HUMAN
Hydrolase (HDase)
DBT Name: Autotaxin (ENPP2)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	D&C red no. 33
Biological Activity	IC50 = 1260 nM (estimated based on the structural similarity with CHEMBL1091752 )					[2]
Structural Similarity	Tanimoto coefficient = 0.911111111
Tested Species	Homo sapiens (Human)
UniProt ID	ENPP2_HUMAN
Potential-driven transporter (PDT)
DBT Name: Solute carrier SLCO2B1 (OATPB)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment (1) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	D&C red no. 33
Biological Activity	Inhibition ratio = 60.9 % (tested by experiment)					[3]
Tested Species	Homo sapiens (Human)
UniProt ID	SO2B1_HUMAN
Experiment (2) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	D&C red no. 33
Biological Activity	Ki = 58.1 uM (tested by experiment)					[3]
Tested Species	Homo sapiens (Human)
UniProt ID	SO2B1_HUMAN

References
1	Effects of commonly used excipients on the expression of CYP3A4 in colon and liver cells. Pharm Res. 2010 Aug;27(8):1703-12.
2	Pharmacophore development and application toward the identification of novel, small-molecule autotaxin inhibitors. J Med Chem. 2010 Apr 22; 53(8):3095-105.
3	Bacterial metabolism rescues the inhibition of intestinal drug absorption by food and drug additives. Proc Natl Acad Sci U S A. 2020 Jul 7;117(27):16009-16018.

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