General Information of DFM (ID: F02778)
Name	Buprenorphine 8 mg tablet
Company	Actavis Pharma
Active Pharmaceutical Ingredient (API)	Buprenorphine		API Info click to show the detail info of this API
	Opiate dependence		ICD-11: 6C43	Approved
Drug Inactive Ingredient (DIGs)
	DIG ID	DIG Info	DIG Name	DIG Functional Class
	E0F6AN	DIG Info	Kyselina citronova	Acidulant ...
	E00DHW	DIG Info	Lactose monohydrate	Binding agent ...
	E01UTP	DIG Info	Magnesium stearate	lubricant
	E0Q6DQ	DIG Info	Mannitol	Diluent ...
	E0NY5F	DIG Info	Sodium citrate dihydrate	Alkalizing agent ...
	E0L0MR	DIG Info	Sunset yellow FCF	Colorant
	E0FU1S	DIG Info	Vinylpyrrolidone	Binding agent ...

Full List of Biological Targets of DIG (DBTs) Regulated by DIG(s) in This DFM
Oxidoreductase (ORase)
DBT Name: N1-methyl adenine demethylase (FTO)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Kyselina citronova
Biological Activity	IC50 = 300000 nM (tested by experiment)					[1]
Tested Species	Homo sapiens (Human)
UniProt ID	FTO_HUMAN
DBT Name: RNA demethylase ALKBH5 (ALKB5)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Kyselina citronova
Biological Activity	IC50 = 488000 nM (tested by experiment)					[2]
Tested Species	Homo sapiens (Human)
UniProt ID	ALKB5_HUMAN
DBT Name: Albendazole monooxygenase (CYP3A4)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Magnesium stearate
Biological Activity	Protein expression downregulation (tested by experiment)					[3]
Tested Species	Homo sapiens (Human)
UniProt ID	CP3A4_HUMAN
Potential-driven transporter (PDT)
DBT Name: Solute carrier SLCO2B1 (OATPB)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment (1) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Sunset yellow FCF
Biological Activity	Inhibition ratio = 54.5 % (tested by experiment)					[4]
Tested Species	Homo sapiens (Human)
UniProt ID	SO2B1_HUMAN
Experiment (2) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Sunset yellow FCF
Biological Activity	Ki = 68.4 uM (tested by experiment)					[4]
Tested Species	Homo sapiens (Human)
UniProt ID	SO2B1_HUMAN
Experiment (3) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Sunset yellow FCF
Biological Activity	Ki = 74 uM (tested by experiment)					[5]
Tested Species	Homo sapiens (Human)
UniProt ID	SO2B1_HUMAN
Transmembrane channel/porin (TC/P)
DBT Name: Glycine receptor alpha-1 (GLRA1)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Mannitol
Biological Activity	EC50 = 12589.25 nM (estimated based on the structural similarity with CHEMBL604608 )					[6]
Structural Similarity	Tanimoto coefficient = 0.804878049
Tested Species	Homo sapiens (Human)
UniProt ID	GLRA1_HUMAN
Nuclear receptor (NR)
DBT Name: Factor HNF-4-alpha (HNF4A)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Kyselina citronova
Biological Activity	IC50 = 39869 nM (tested by experiment)					[7]
Tested Species	Homo sapiens (Human)
UniProt ID	HNF4A_HUMAN
Other protein families (OPF)
DBT Name: Perilipin-1 (PLIN1)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Kyselina citronova
Biological Activity	IC50 = 3708 nM (tested by experiment)					[7]
Tested Species	Homo sapiens (Human)
UniProt ID	PLIN1_HUMAN
DBT Name: Thrombopoietin receptor (MPL)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Sunset yellow FCF
Biological Activity	EC50 = 3000 nM (estimated based on the structural similarity with CHEMBL122867 )					[8]
Structural Similarity	Tanimoto coefficient = 0.93081761
Tested Species	Homo sapiens (Human)
UniProt ID	TPOR_HUMAN

References
1	Structural basis for inhibition of the fat mass and obesity associated protein (FTO). J Med Chem. 2013 May 9; 56(9):3680-8.
2	Repair of methyl lesions in RNA by oxidative demethylation. Med Chem Comm. (2014) 5:1797-1803.
3	Effects of commonly used excipients on the expression of CYP3A4 in colon and liver cells. Pharm Res. 2010 Aug;27(8):1703-12.
4	Bacterial metabolism rescues the inhibition of intestinal drug absorption by food and drug additives. Proc Natl Acad Sci U S A. 2020 Jul 7;117(27):16009-16018.
5	The activities of drug inactive ingredients on biological targets. Science. 2020 Jul 24;369(6502):403-413.
6	Pharmacological property optimization for allosteric ligands: A medicinal chemistry perspective. Bioorg Med Chem Lett. 2017 Jun 1; 27(11):2239-2258.
7	PubChem BioAssay data set.
8	Hydrazinonaphthalene and azonaphthalene thrombopoietin mimics are nonpeptidyl promoters of megakaryocytopoiesis. J Med Chem. 2001 Oct 25; 44(22):3730-45.

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