General Information of DFM (ID: F02628)
Name	Bisacodyl 5 mg tablet
Company	Physicians Total Care
Active Pharmaceutical Ingredient (API)	Bisacodyl		API Info click to show the detail info of this API
	Irritable bowel syndrome		ICD-11: DD91	Approved
Drug Inactive Ingredient (DIGs)
	DIG ID	DIG Info	DIG Name	DIG Functional Class
	E0SJ4H	DIG Info	Beta-D-lactose	Diluent ...
	E0Y4VO	DIG Info	Dibutyl phthalate	Plasticizing agent ...
	E0FH4N	DIG Info	Docusate sodium	Surfactant
	E06WYP	DIG Info	Glycerin	Antimicrobial preservative ...
	E0VW7Y	DIG Info	Kaolin	Adsorbent ...
	E01UTP	DIG Info	Magnesium stearate	lubricant
	E0V6QI	DIG Info	methylparaben	Antimicrobial preservative
	E0Q7IF	DIG Info	Povidone	Binding agent ...
	E0KP0P	DIG Info	Propyl 4-hydroxybenzoate	Antimicrobial preservative
	E0Q3PU	DIG Info	Saccharose	Binding agent ...
	E0Y7PW	DIG Info	Sodium benzoate	Antimicrobial preservative ...
	E0T3FM	DIG Info	Sorbitan monooleate	Dispersing agent ...
	E0QY1F	DIG Info	Talc	Anticaking agent ...
	E05BSD	DIG Info	Titanium dioxide	Coating agent ...

Full List of Biological Targets of DIG (DBTs) Regulated by DIG(s) in This DFM
G-protein coupled receptor (GPCR)
DBT Name: Muscarinic receptor M1 (ACM1)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Propyl 4-hydroxybenzoate
Biological Activity	IC50 = 23 uM (tested by experiment)					[1]
Tested Species	Homo sapiens (Human)
UniProt ID	ACM1_HUMAN
Oxidoreductase (ORase)
DBT Name: Prostaglandin G/H synthase 1 (COX-1)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Propyl 4-hydroxybenzoate
Biological Activity	IC50 > 3 uM (tested by experiment)					[1]
Tested Species	Homo sapiens (Human)
UniProt ID	PGH1_HUMAN
DBT Name: Prostaglandin G/H synthase 2 (COX-2)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Propyl 4-hydroxybenzoate
Biological Activity	IC50 > 3 uM (tested by experiment)					[1]
Tested Species	Homo sapiens (Human)
UniProt ID	PGH2_HUMAN
DBT Name: Albendazole monooxygenase (CYP3A4)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment (1) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Magnesium stearate
Biological Activity	Protein expression downregulation (tested by experiment)					[2]
Tested Species	Homo sapiens (Human)
UniProt ID	CP3A4_HUMAN
Experiment (2) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Docusate sodium
Biological Activity	IC50 = 65 uM (tested by experiment)					[3]
Tested Species	Homo sapiens (Human)
UniProt ID	CP3A4_HUMAN
Experiment (3) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Povidone
Biological Activity	IC50 = 107 uM (tested by experiment)					[3]
Tested Species	Homo sapiens (Human)
UniProt ID	CP3A4_HUMAN
Experiment (4) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Povidone
Biological Activity	Protein expression upregulation (tested by experiment)					[2]
Tested Species	Homo sapiens (Human)
UniProt ID	CP3A4_HUMAN
DBT Name: Cytochrome P450 2E1 (CYP2E1)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Docusate sodium
Biological Activity	EC50 = 141.8 uM (tested by experiment)					[3]
Tested Species	Homo sapiens (Human)
UniProt ID	CP2E1_HUMAN
DBT Name: Cytochrome P450 2C9 (CYP2C9)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Docusate sodium
Biological Activity	EC50 = 203.3 uM (tested by experiment)					[3]
Tested Species	Homo sapiens (Human)
UniProt ID	CP2C9_HUMAN
DBT Name: Mephenytoin 4-hydroxylase (CYP2C19)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Docusate sodium
Biological Activity	IC50 = 372 uM (tested by experiment)					[3]
Tested Species	Homo sapiens (Human)
UniProt ID	CP2CJ_HUMAN
DBT Name: Debrisoquine 4-hydroxylase (CYP2D6)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Docusate sodium
Biological Activity	IC50 = 95.5 uM (tested by experiment)					[3]
Tested Species	Homo sapiens (Human)
UniProt ID	CP2D6_HUMAN
DBT Name: Cytochrome P450 3A5 (CYP3A5)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Docusate sodium
Biological Activity	EC50 = 195.8 uM (tested by experiment)					[3]
Tested Species	Homo sapiens (Human)
UniProt ID	CP3A5_HUMAN
Transferase (TFase)
DBT Name: VEGF-2 receptor (KDR)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Propyl 4-hydroxybenzoate
Biological Activity	IC50 = 24 uM (tested by experiment)					[1]
Tested Species	Homo sapiens (Human)
UniProt ID	VGFR2_HUMAN
Hydrolase (HDase)
DBT Name: Cathepsin B (CTSB)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Dibutyl phthalate
Biological Activity	IC50 = 230000 nM (tested by experiment)					[4]
Tested Species	Mus musculus (Mouse)
UniProt ID	CATB_MOUSE
DBT Name: Histone deacetylase (HDAC)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Docusate sodium
Biological Activity	IC50 = 9600 nM (estimated based on the structural similarity with CHEMBL271677 )					[5]
Structural Similarity	Tanimoto coefficient = 0.824561404
Tested Species	Homo sapiens (Human)
UniProt ID	HDAC1_HUMAN ; HDAC2_HUMAN ; HDAC3_HUMAN ; HDAC4_HUMAN ; HDAC5_HUMAN ; HDAC6_HUMAN ; HDAC7_HUMAN ; HDAC8_HUMAN ; HDAC9_HUMAN
DBT Name: Leukotriene A-4 hydrolase (LTA4H)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Propyl 4-hydroxybenzoate
Biological Activity	Ki = 260 uM (tested by experiment)					[1]
Tested Species	Homo sapiens (Human)
UniProt ID	LKHA4_HUMAN
Lyase/isomerase/ligase (L/I/G)
DBT Name: Carbonic anhydrase I (CA1)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	methylparaben
Biological Activity	Ki = 2600 nM (tested by experiment)					[6]
Tested Species	Homo sapiens (Human)
UniProt ID	CAH1_HUMAN
DBT Name: Carbonic anhydrase II (CA2)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment (1) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	methylparaben
Biological Activity	Ki = 3400 nM (tested by experiment)					[6]
Tested Species	Homo sapiens (Human)
UniProt ID	CAH2_HUMAN
Experiment (2) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Sodium benzoate
Biological Activity	Ki = 30000 nM (tested by experiment)					[7]
Tested Species	Homo sapiens (Human)
UniProt ID	CAH2_HUMAN
DBT Name: Carbonic anhydrase IV (CA4)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Sodium benzoate
Biological Activity	Ki = 74700 nM (tested by experiment)					[7]
Tested Species	Homo sapiens (Human)
UniProt ID	CAH4_HUMAN
DBT Name: Carbonic anhydrase IX (CA9)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	methylparaben
Biological Activity	Ki = 5500 nM (tested by experiment)					[6]
Tested Species	Homo sapiens (Human)
UniProt ID	CAH9_HUMAN
DBT Name: Carbonic anhydrase VII (CA7)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	methylparaben
Biological Activity	Ki = 780 nM (tested by experiment)					[6]
Tested Species	Homo sapiens (Human)
UniProt ID	CAH7_HUMAN
DBT Name: Carbonic anhydrase XII (CA12)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	methylparaben
Biological Activity	Ki = 820 nM (tested by experiment)					[6]
Tested Species	Homo sapiens (Human)
UniProt ID	CAH12_HUMAN
DBT Name: Carbonic anhydrase XIV (CA14)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	methylparaben
Biological Activity	Ki = 4100 nM (tested by experiment)					[6]
Tested Species	Homo sapiens (Human)
UniProt ID	CAH14_HUMAN
DBT Name: Cholesterol 25-hydroxylase (CYP1A2)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment (1) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Docusate sodium
Biological Activity	EC50 = 147.4 uM (tested by experiment)					[3]
Tested Species	Homo sapiens (Human)
UniProt ID	CP1A2_HUMAN
Experiment (2) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Povidone
Biological Activity	IC50 = 78.3 uM (tested by experiment)					[3]
Tested Species	Homo sapiens (Human)
UniProt ID	CP1A2_HUMAN
Potential-driven transporter (PDT)
DBT Name: Norepinephrine transporter (NET)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Propyl 4-hydroxybenzoate
Biological Activity	IC50 = 30 uM (tested by experiment)					[1]
Tested Species	Homo sapiens (Human)
UniProt ID	SC6A2_HUMAN
DBT Name: Solute carrier SLCO2B1 (OATPB)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment (1) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Propyl 4-hydroxybenzoate
Biological Activity	Inhibition ratio = 25.1 % (tested by experiment)					[8]
Tested Species	Homo sapiens (Human)
UniProt ID	SO2B1_HUMAN
Experiment (2) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Propyl 4-hydroxybenzoate
Biological Activity	Ki = 200 uM (tested by experiment)					[1]
Tested Species	Homo sapiens (Human)
UniProt ID	SO2B1_HUMAN
Experiment (3) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Docusate sodium
Biological Activity	Inhibition ratio = 87.8 % (tested by experiment)					[8]
Tested Species	Homo sapiens (Human)
UniProt ID	SO2B1_HUMAN
Experiment (4) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Docusate sodium
Biological Activity	Ki = 2.76 uM (tested by experiment)					[8]
Tested Species	Homo sapiens (Human)
UniProt ID	SO2B1_HUMAN
Experiment (5) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Docusate sodium
Biological Activity	Ki = 2.3 uM (tested by experiment)					[1]
Tested Species	Homo sapiens (Human)
UniProt ID	SO2B1_HUMAN
Nuclear receptor (NR)
DBT Name: Androgen receptor (AR)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment (1) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Propyl 4-hydroxybenzoate
Biological Activity	IC50 = 309029.54 nM (tested by experiment)					[9]
Tested Species	Rattus norvegicus (Rat)
UniProt ID	ANDR_RAT
Experiment (2) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	methylparaben
Biological Activity	IC50 > 426579.52 nM (tested by experiment)					[9]
Tested Species	Rattus norvegicus (Rat)
UniProt ID	ANDR_RAT
DBT Name: Estrogen receptor alpha (ESR1)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Propyl 4-hydroxybenzoate
Biological Activity	IC50 = 11 uM (tested by experiment)					[1]
Tested Species	Homo sapiens (Human)
UniProt ID	ESR1_HUMAN
Cell line (CELL)
DBT Name: Lung cancer A-549 cells (A-549)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment (1) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	methylparaben
Biological Activity	IC50 > 20 ug.mL-1 (tested by experiment)					[10]
Tested Species	Homo sapiens (Human)
Cellosaurus ID	CVCL_0023
Experiment (2) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Docusate sodium
Biological Activity	IC50 = 154000 nM (estimated based on the structural similarity with CHEMBL271677 )					[5]
Structural Similarity	Tanimoto coefficient = 0.824561404
Tested Species	Homo sapiens (Human)
Cellosaurus ID	CVCL_0023
DBT Name: Hepatoblastoma HepG2 cells (HepG2)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	methylparaben
Biological Activity	IC50 > 20 ug.mL-1 (tested by experiment)					[10]
Tested Species	Homo sapiens (Human)
Cellosaurus ID	CVCL_0027
DBT Name: Cervical cancer KB cells (KB)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	methylparaben
Biological Activity	IC50 > 200000 nM (tested by experiment)					[11]
Tested Species	Homo sapiens (Human)
Cellosaurus ID	CVCL_0372
DBT Name: Carcinoma MDA-MB-231 cells (MDA-MB-231)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	methylparaben
Biological Activity	IC50 > 20 ug.mL-1 (tested by experiment)					[10]
Tested Species	Homo sapiens (Human)
Cellosaurus ID	CVCL_0062

References
1	The activities of drug inactive ingredients on biological targets. Science. 2020 Jul 24;369(6502):403-413.
2	Effects of commonly used excipients on the expression of CYP3A4 in colon and liver cells. Pharm Res. 2010 Aug;27(8):1703-12.
3	Mediation of in vitro cytochrome p450 activity by common pharmaceutical excipients. Mol Pharm. 2013 Jul 1;10(7):2739-48.
4	Cathepsin B inhibitory activities of phthalates isolated from a marine Pseudomonas strain. Bioorg Med Chem Lett. 2008 Mar 15; 18(6):2083-8.
5	New sulfurated derivatives of valproic acid with enhanced histone deacetylase inhibitory activity. Bioorg Med Chem Lett. 2008 Mar 15; 18(6):1893-7.
6	Mono-/dihydroxybenzoic acid esters and phenol pyridinium derivatives as inhibitors of the mammalian carbonic anhydrase isoforms I, II, VII, IX, XII and XIV. Bioorg Med Chem. 2013 Mar 15; 21(6):1564-9.
7	Carbonic anhydrase inhibitors. Interaction of isozymes I, II, IV, V, and IX with carboxylates. Bioorg Med Chem Lett. 2005 Feb 1; 15(3):573-8.
8	Bacterial metabolism rescues the inhibition of intestinal drug absorption by food and drug additives. Proc Natl Acad Sci U S A. 2020 Jul 7;117(27):16009-16018.
9	Impact of induced fit on ligand binding to the androgen receptor: a multidimensional QSAR study to predict endocrine-disrupting effects of environmental chemicals. J Med Chem. 2005 Sep 8; 48(18):5666-74.
10	Bioactive Phenolic Components from the Twigs of Atalantia buxifolia. J Nat Prod. 2018 Jul 27; 81(7):1534-1539.
11	Synthesis and biological evaluation of 12 allenic aromatic ethers. Bioorg Med Chem Lett. 2007 May 15; 17(10):2785-8.

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