General Information of DFM (ID: F23768)
Name	Halobetasol Propionate 0.05% lotion
Company	Sun Pharmaceutical Industries
Active Pharmaceutical Ingredient (API)	Halobetasol propionate		API Info click to show the detail info of this API
				Approved
Drug Inactive Ingredient (DIGs)
	DIG ID	DIG Info	DIG Name	DIG Functional Class
	E05GFF	DIG Info	Butylparaben	Antimicrobial preservative
	E07AGB	DIG Info	Cetyl alcohol	Coating agent ...
	E09IIR	DIG Info	Diisopropyl adipate	Emollient
	E06WYP	DIG Info	Glycerin	Antimicrobial preservative ...
	E0QA6Q	DIG Info	Octyldodecanol	Emollient ...
	E0E7MW	DIG Info	Poloxamer 407	Emulsifying agent ...
	E0G3NO	DIG Info	Polyethylene glycol 4000	Coating agent ...
	E01RHD	DIG Info	Polyoxyl 20 cetyl ether	Emulsifying agent ...
	E09BVZ	DIG Info	Propylene	Other agent
	E0X6QO	DIG Info	Propylparaben sodium	Antimicrobial preservative
	E0L2PN	DIG Info	Sodium hydroxide	Alkalizing agent
	E0XV1Z	DIG Info	Stearyl alcohol	Emollient ...
	E0H2SC	DIG Info	Water	Solvent

Full List of Biological Targets of DIG (DBTs) Regulated by DIG(s) in This DFM
G-protein coupled receptor (GPCR)
DBT Name: Muscarinic receptor M1 (ACM1)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Butylparaben
Biological Activity	IC50 = 12 uM (tested by experiment)					[1]
Tested Species	Homo sapiens (Human)
UniProt ID	ACM1_HUMAN
DBT Name: Alpha-1A adrenoceptor (ADA1A)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Butylparaben
Biological Activity	IC50 = 16.4 uM (tested by experiment)					[1]
Tested Species	Homo sapiens (Human)
UniProt ID	ADA1A_HUMAN
DBT Name: Thromboxane A2 receptor (TBXA2R)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Butylparaben
Biological Activity	IC50 = 19 uM (tested by experiment)					[1]
Tested Species	Homo sapiens (Human)
UniProt ID	TA2R_HUMAN
Oxidoreductase (ORase)
DBT Name: Prostaglandin G/H synthase 2 (COX-2)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Butylparaben
Biological Activity	IC50 > 3 uM (tested by experiment)					[1]
Tested Species	Homo sapiens (Human)
UniProt ID	PGH2_HUMAN
DBT Name: Albendazole monooxygenase (CYP3A4)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment (1) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Poloxamer 407
Biological Activity	EC50 = 32.8 uM (tested by experiment)					[2]
Tested Species	Homo sapiens (Human)
UniProt ID	CP3A4_HUMAN
Experiment (2) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Polyethylene glycol 4000
Biological Activity	Inhibition ratio < 40 % (tested by experiment)					[3]
Tested Species	Homo sapiens (Human)
UniProt ID	CP3A4_HUMAN
DBT Name: Cytochrome P450 2E1 (CYP2E1)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment (1) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Polyoxyl 20 cetyl ether
Biological Activity	EC50 = 247.1 uM (tested by experiment)					[2]
Tested Species	Homo sapiens (Human)
UniProt ID	CP2E1_HUMAN
Experiment (2) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Poloxamer 407
Biological Activity	IC50 = 218.9 uM (tested by experiment)					[2]
Tested Species	Homo sapiens (Human)
UniProt ID	CP2E1_HUMAN
DBT Name: Cytochrome P450 2C9 (CYP2C9)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment (1) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Polyoxyl 20 cetyl ether
Biological Activity	EC50 = 391.4 uM (tested by experiment)					[2]
Tested Species	Homo sapiens (Human)
UniProt ID	CP2C9_HUMAN
Experiment (2) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Poloxamer 407
Biological Activity	EC50 = 355 uM (tested by experiment)					[2]
Tested Species	Homo sapiens (Human)
UniProt ID	CP2C9_HUMAN
DBT Name: Debrisoquine 4-hydroxylase (CYP2D6)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment (1) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Polyoxyl 20 cetyl ether
Biological Activity	IC50 = 242.8 uM (tested by experiment)					[2]
Tested Species	Homo sapiens (Human)
UniProt ID	CP2D6_HUMAN
Experiment (2) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Poloxamer 407
Biological Activity	EC50 = 101.6 uM (tested by experiment)					[2]
Tested Species	Homo sapiens (Human)
UniProt ID	CP2D6_HUMAN
Lyase/isomerase/ligase (L/I/G)
DBT Name: Cholesterol 25-hydroxylase (CYP1A2)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment (1) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Diisopropyl adipate
Biological Activity	IC50 = 110000 nM (estimated based on the structural similarity with CHEMBL365740 )					[4]
Structural Similarity	Tanimoto coefficient = 0.818181818
Tested Species	Homo sapiens (Human)
UniProt ID	CP1A2_HUMAN
Experiment (2) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Polyoxyl 20 cetyl ether
Biological Activity	EC50 = 211.5 uM (tested by experiment)					[2]
Tested Species	Homo sapiens (Human)
UniProt ID	CP1A2_HUMAN
Potential-driven transporter (PDT)
DBT Name: Dopamine transporter (DAT)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Butylparaben
Biological Activity	IC50 = 19 uM (tested by experiment)					[1]
Tested Species	Homo sapiens (Human)
UniProt ID	SC6A3_HUMAN
DBT Name: Norepinephrine transporter (NET)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Butylparaben
Biological Activity	IC50 = 20 uM (tested by experiment)					[1]
Tested Species	Homo sapiens (Human)
UniProt ID	SC6A2_HUMAN
DBT Name: Solute carrier SLCO2B1 (OATPB)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment (1) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Butylparaben
Biological Activity	Inhibition ratio = 73.5 % (tested by experiment)					[5]
Tested Species	Homo sapiens (Human)
UniProt ID	SO2B1_HUMAN
Experiment (2) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Butylparaben
Biological Activity	Ki = 44.3 uM (tested by experiment)					[5]
Tested Species	Homo sapiens (Human)
UniProt ID	SO2B1_HUMAN
DBT Name: Monoamine transporter VAT2 (VMAT2)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Butylparaben
Biological Activity	Ki = 6.6 uM (tested by experiment)					[1]
Tested Species	Homo sapiens (Human)
UniProt ID	VMAT2_HUMAN
Transmembrane channel/porin (TC/P)
DBT Name: Sodium channel alpha Nav1.5 (SCN5A)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Butylparaben
Biological Activity	Ki = 33 uM (tested by experiment)					[1]
Tested Species	Homo sapiens (Human)
UniProt ID	SCN5A_HUMAN
Nuclear receptor (NR)
DBT Name: Estrogen receptor alpha (ESR1)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment (1) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Butylparaben
Biological Activity	IC50 = 1420 nM (tested by experiment)					[6]
Tested Species	Homo sapiens (Human)
UniProt ID	ESR1_HUMAN
Experiment (2) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Propylparaben sodium
Biological Activity	EC50 = 38200 nM (estimated based on the structural similarity with CHEMBL15841 )					[7]
Structural Similarity	Tanimoto coefficient = 0.946428571
Tested Species	Homo sapiens (Human)
UniProt ID	ESR1_HUMAN
Experiment (3) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Butylparaben
Biological Activity	IC50 = 3.5 uM (tested by experiment)					[1]
Tested Species	Homo sapiens (Human)
UniProt ID	ESR1_HUMAN
DBT Name: Oxysterols receptor LXR-beta (NR1H2)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Butylparaben
Biological Activity	Ki = 66000 nM (tested by experiment)					[8]
Tested Species	Homo sapiens (Human)
UniProt ID	NR1H2_HUMAN
Cell line (CELL)
DBT Name: Colon cancer Caco-2 cells (Caco-2)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment (1) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Poloxamer 407
Biological Activity	Inhibition ratio = 38 % (tested by experiment)					[9]
Tested Species	Homo sapiens (Human)
Experiment (2) for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Poloxamer 407
Biological Activity	Inhibition ratio > 45 % (tested by experiment)					[10]
Tested Species	Homo sapiens (Human)
DBT Name: Breast cancer FM3A cells (FM3A)				Click to Show/Hide
Detailed Information	DBT Info click to show the detail info of this DBT
Experiment for Assessing the Biological Activity of the Studied DIG on This DBT
Interacting DIG	Cetyl alcohol
Biological Activity	EC50 = 1600 nM (estimated based on the structural similarity with CHEMBL28459 )					[11]
Structural Similarity	Tanimoto coefficient = 0.861111111
Tested Species	Mus musculus (Mouse)
Cellosaurus ID	CVCL_3869

References
1	The activities of drug inactive ingredients on biological targets. Science. 2020 Jul 24;369(6502):403-413.
2	Mediation of in vitro cytochrome p450 activity by common pharmaceutical excipients. Mol Pharm. 2013 Jul 1;10(7):2739-48.
3	Pharmaceutical excipients inhibit cytochrome P450 activity in cell free systems and after systemic administration. Eur J Pharm Biopharm. 2008 Sep;70(1):279-88.
4	Predictive three-dimensional quantitative structure-activity relationship of cytochrome P450 1A2 inhibitors. J Med Chem. 2005 Jun 2; 48(11):3808-15.
5	Bacterial metabolism rescues the inhibition of intestinal drug absorption by food and drug additives. Proc Natl Acad Sci U S A. 2020 Jul 7;117(27):16009-16018.
6	Disconnecting the Estrogen Receptor Binding Properties and Antimicrobial Properties of Parabens through 3,5-Substitution. ACS Med Chem Lett. 2017 Dec 15; 9(1):51-55.
7	Discovery of natural estrogen receptor modulators with structure-based virtual screening. Bioorg Med Chem Lett. 2013 Jun 1; 23(11):3329-33.
8	Identify liver X receptor modulator building blocks by developing a fluorescence polarization-based competition assay. Eur J Med Chem. 2019 Sep 15; 178:458-467.
9	Inhibition of human efflux transporter ABCC2 (MRP2) by self-emulsifying drug delivery system: influences of concentration and combination of excipients. J Pharm Pharm Sci. 2014;17(4):447-60.
10	Interactions between human multidrug resistance related protein (MRP2; ABCC2) and excipients commonly used in self-emulsifying drug delivery systems (SEDDS). Int J Pharm. 2013 Apr 15;447(1-2):192-8.
11	Synthesis and notable antimalarial activity of acyclic peroxides, L-(alkyldioxy)-L-(methyldioxy)cyclododecanes. J Med Chem. 2002 Mar 14; 45(6):1374-8.

If you find any error in data or bug in web service, please kindly report it to Dr. Zhang and Dr. Mou.